Gel.ProCCD Gel Documentation System
Sharp zoom lens coupled to a zero defect, Sony scientific grade CCD or CMOS camera; the Gel.ProCCD geldocumentation system, is a high quality, yet low cost professional gel and bio-imaging system. Options fromagarose, PAGE, western blots, dot blots, nucleic acid, colorimetric to fluorescent & chemifluorescent imaging,Colonies, TLC, ELISA Plates, Petri Dish etc. and upgradeable to chemiluminescence.
Featuring high resolution extremely sensitive 16-bit scientific grade CCD camera (with choice of 1.4, 5.6, 6.3 and 9 megapixel cameras) with extremely low noise and dynamic flat field calibration coupled to a C-mountmotorized zoom lens (with option for numerical feedback value) that is pre-focussed for different zooms orsample setting, mounted inside an enclosure that covers and protects the camera and lens. The camera, lens &cabinet connects to & can be controlled from the PC using live-view acquisition capture software.
The mild & stainless steel, epoxy coated microprocessor controlled dark room cabinet has features for UV irradiation safety switch that switches off the UV lights as soon as the door is opened. Additionally, a timer is provided that switches off UV/White light in case the user forgets to shut it off. Option to adjust timer for excision. Also, the user can opt for automatic switching on of the Dual Epi-White LEDs on opening of door and for automatic controls of UV light and exposure. A transilluminator cooling system is also provided to prevent overheating of gels. Option for a front viewing window (with amber filter) & 2 side access doors for gel observation & cutting. Gel platform temperature control (<34 C) & alignment templates also available.
Choice of illumination options with UV trans/epi lighting, wavelength-specific LEDs and more, add to theversatility of the system. UV roll-out transilluminator can be provided in dual 302-312nm (detection range downto 0.1 ng with EtBr, SYBR etc. & 10 pg with Vistra Green) and 365nm (additionally 254nm, for triple wavelengthconfiguration), and even Red, Green, Blue LEDs ranges. Large filter size of transilluminator for gel images up to26x26cm, with extra large special filter size of up to 28x38cm is also available. Colorimetric samples up to38x30cm can be captured. Epi-white light illumination from above is standard using two LED tubes, with anoption for low (50%) & high (100%) intensity control. Optionally, ultraviolet epi-illumination in a variety ofwavelengths (254/312/365nm) can also be provided. Standard Trans White light and Trans Blue light tables upto25x25 cm are also available with options for sizes up to 30 x 37 cm.
* Please note that 300nm, 302nm, 306nm and 312nm are in the same range of wavelengths called UV-B (also known as mid-range). Some manufacturers quote 302nm but it is the same as 312nm..
Epi-white light illumination through dual LED tubes from above is standard. Ultraviolet epi-illumination in a variety of wavelengths can also be provided. For specific stains, epi-light in blue, green or red LEDs can be provided. A combination of all these illumination modes in the same system can be provided.
- Standard is a manual 3-position filter holder
- Advanced models with 3, 4 and 7-position manual and motorized filter holder are also available
- Orange 590 EtBr and clear filters are standard.
- Orange 590: Ethidium Bromide (EtBr), SYPRO Ruby, ProQ Diamond, Deep Purple, etc
- Green 520: SYBR Green I, Green II, SYBR Gold, GFP, Fluorescein, FITC
- Orange 560: SYBR Safe, Rhodamine, Cy3, etc
- Red 630: SYPRO Red, Texas Red, etc
- Blue 480: Hoechst, Coumarin, etc
- Clear: Coomassie Blue, Silver etc
How the Gel Documentation & Imaging Analysis System works
A transilluminator is mounted on roll-out rails inside a light-tight cabinet. To place the gel, the door is opened and transilluminator pulled out. Push it in, close the door and switch on the UV light and camera. The camera is mounted on top of the cabinet along with zoom lens and wavelenght filter (nominally 590nm filter suitable for Ethidium Bromide EtBr gels is provided).
Connect the camera to the PC using the USB cable and gel capture software, to get a real-time live view image of the gel. Adjust camera settings (like shutter speed, aperture, focus) till the optimum image is obtained. Click your images and save them on the PC. A white light epi-illuminations source is also provided, that illuminates objects from top.
With the optional 1D gel analysis software, you can analyse the gels.
- Dark room cabinet with safety switch with low footprint
- Roll out variable intensity 312nm transilluminator
- Epi-white light illumination
- CCD camera
- Gel capture software
- 590nm orange filter, suitable for EtBr stained gels
- Power cords, USB cable, instruction manual, warranty card
- Options that can be ordered like motorized filter holder, special zoom lens, illumination etc
- 1 year standard warranty against manufacturing defects
Gel Capture Software
Gel Analysis Software
Gel image acquisition and control software has full function control panel which allows user to adjust camera, lens settings like exposure time settings, motorized zoom, focus and aperture (iris). Histogram display on the screen shows image saturation and indicates over-saturation/exposure.
Options for manual exposure, auto exposure and exposure for intense & faint bands are available. Application protocols can be further edited/created and saved with specific controls for light source, camera settings, exposures, aperture etc. The user can opt to choose the manual mode or automatic mode of light source, focus, exposure & aperture. Option for Manual or Motorized filter wheel with Triple, 4 or 7 filter slots are available.